By Rifei Chen

For the linear backbone were so few and the backbone of BBa_J04450 was pSB1C3, we decide to cut it by XbaI and PstI restriction enzyme and recycle the about 2000bp vector as the backbone for biobricks. Of course, one advantage to make it as backbone is that we can recognize whether the gene we designed have inserted. Because if not, the bacterium will express red chromoprotein which one we unnecessary to pick up.

2014/08/22
Transfect the backbone of biobricks