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From 2014.igem.org

Results / Heavy metals / Arabidopsis thaliana T4MBP

Labwork

Transformation of A. thaliana with our T4MBP:

1. Exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol.
2. We integrated our T4MBP in our modified pORE_E3_2x35S.
3. Application of our floral dip method.
4. After 6 weeks we harvested transgenic seeds from A. thaliana and plated these seeds on selection MSO-media.
5. At last we potted transformed plants.

Results

We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.