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From 2014.igem.org

 
 

A photo-induction box was constructed using blue LEDs (light emitting diodes) and foamboard obtained from garbage. The device was designed such that a each well of a 24-well plate is fully covered by the light-conus of one LED. When using glass-bottom microscopy plates this should enable the simultaneous induction (or not-induction) at different intensities and an easy readout in one single flow cytometry assay. Alternatively, a separate plate can be wrapped in aluminium foil to keep it in the dark.

General Data

• Wavelength: 460-470nm

• Format of cultures: Best used with 24-well plates

List of meterials used

• 24x LEDs from highlight-led.de (No. 75000001;50), LED 5mm wasserklar blau 12.000mcd 20°, 460-470nm 2.9-4.0V 20-30mA

• foambord (dark colour in order to prevent light from shining through)

• 96-well plate

• Aluminium foil (to maximise irradiation through reflection inside the box and to prevent influence from the outside)