Team:WashU StLouis

Nitrogen Fixation in E. coli

Two Key Components to our Project:

Our team is using genes from cyanobacteria to get nitrogen fixation working in E. coli and is working to regulate the transcription of these nitrogen fixation genes with light

How does your project work?

Right now, our plan is to divide and conquer. Ben and Jeffrey are working in Dr. Tae Seok Moon's lab with Cheryl Immethun on the transcription regulation project while Richard and Caroline are working in Dr. Himadri Pakrasi's lab with Dr. Deng Liu, Bert Berla, and Andrew Ng on getting the cyanobacterial nif cluster to function in E. coli . We are looking to study how these nitrogen fixation genes function in a foreign environment and to generate a light-senstive transcription system; our end goal is to unite these two projects to create a system that fixes nitrogen only in the absence of light.

Who will your project help?

Our project is the first step of a much larger, much more complex endeavor. Nitrogen overabundance and nitrogen depletion are simultaneously big stumbling blocks in modern agriculture. The solution to both of these problems would be to endow plants themselves with the ability to fix nitrogen so that they could autonomously supply their own nitrogen for proteins, DNA, etc. We are taking the first step towards this ambitious goal by studying how the genes for nitrogen fixation from Cyanothece sp. ATTC 51142 work in different environments and constructing an artificial transcriptional system. We are currently working in E. coli because it is easy to engineer, but the next step would be to move into a cyanobacteria such as Synechocystis sp. PCC 6803. We hope that by making these initial steps that we may be helping to pave the way for future research that may put an end to world hunger.

Why did you choose this project?

What motivated you to work on this project? Tell us what inspires you.