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Team:Brasil-SP/Project/ResponseModule
From 2014.igem.org
Response Module
The reporter gene chosen for the output system was the GFP. The main reason for that choice was the simplicity in measuring its fluorescenchttps://2014.igem.org/Main_Pagee with the fluorimeter and flow cytometry, but it can be replaced by any other reporter system. In the final development stage of the project we envision a output that do not need to be excited like the GFP does.
Note that the biossensor will perform a negative detection. In other words, when the patient is healthy we'll have no fluorescence, and when in a unhealthy situation the bacteria will not glow. In a more realistic approach, we do not expect our system to have only two intensities (zero or maximum glow). What might happen is that we'll have a more continuous spectrum of intensities, which would make it harder to differentiate between a negative and positive diagnosis. To deal with this problem we are adding a negative control in our microfluidic device. In this negative chamber there will be no blood serum in contact with our bacteria, therefore cathepsin S will work at full potencial generating a maximum LasR production and consequently maximum fluorescence.
