Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate

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Curli Module - Cloning - Golden Gate Assembly

To construct the BioBricks BBa_K1316013 and BBa_K1316014 , encoding a rhamnose promoter and the coding sequences of csgB and csgA or csgA-HIS, we ended up having 3 pieces of DNA to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks (see figure 1).

Figure 1. **Golden Gate Assembly of BBa_K1316013 and BBa_K1316014 .** The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of *csgB* and *csgA* or *csgA-HIS*, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.