Team:Bielefeld-CeBiTec/Results/CO2-fixation

From 2014.igem.org

Revision as of 05:52, 15 October 2014 by Bpucker (Talk | contribs)


CO2 fixation

The second module aims to change E. coli in a way that it binds carbon dioxide. This changes this bacterium from heterotroph to autotroph. To achieve this we want to establish the Calvin-cycle. E. coli has all enzymes for the Calvin-cycle except of three.


Missing enzymes in Calvin-cycle
The different results for all three enzymes are mentioned in the Calvin-cycle section. One important step for the carbon dioxide fixation is the RuBisCO (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E. coli we decided to transform genes for the carboxysome because of its properties.

Here you will find information about the execution of our experiments.

Retrieved from "http://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation"