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Team:UT-Tokyo/CTCD/Content
From 2014.igem.org
EGP-2 promoter
EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.
EpCAM is a trans membrane glycoprotein which is expressed in epithelial cells and shows high level expression in various type of human epithelial carcinomas[2].Using this promoter, reporter genes can be expressed only in cancer cells.
miRNA-142
MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[3].Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.
We focused on a tissue-specific promoter and miRNA in order to detect CTCs.
As well as hematopoietic cells, many other tissues shows specific miRNA expression patterns[3]. Therefore, if by making a circuit that returns an output to a certain miRNA expression pattern, we may identify where a CTC comes from.
In addition to the detection of CTCs, combinations of tissue-specific promoter and miRNA can be applied to many treatments. For example, suicide genes can be expressed in cancer cells in a specific tissue.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
