Our team submitted a total of 54 Biobricks (biobricks 51 and 52 do not exist).
| Biobrick |
What it is |
Function |
Why do we use it? |
Group |
| BBa_K1486000 |
CpxR coding sequence |
Transcription factor |
To make most of our biobricks! |
Bacteria |
| BBa_K1486001 |
CpxR under arabinose promoter |
Treanscription factor |
|
Bacteria |
| BBa_K1486002 |
PAra + sfGFP CpxR [Nterm] |
Expresses fused protein |
Test CpxR expression & Ara promoter |
Bacteria |
| BBa_K1486003 |
Flexible linker |
Attaches two proteins together |
|
Bacteria |
| BBa_K1486004 |
Flexible linker |
Attaches two proteins together |
|
Bacteria |
| BBa_K1486005 |
PAra + CpxR sfGFP [Cterm] |
Expresses fused protein |
Test CpxR expression & Ara promoter |
Bacteria |
| BBa_K1486006 |
IFP[1] |
N terminus of split IFP |
|
Bacteria |
| BBa_K1486007 |
IFP[2] |
C terminus of split IFP |
|
Bacteria |
| BBa_K1486008 |
CxpR & Split IFP1.4 [Cterm + Cterm] |
Two CpxR CDS, each C terminus attached to a moiety of IFP |
Characterize CpxR dimerization |
Bacteria |
| BBa_K1486009 |
CxpR & Split IFP1.4 [Nterm + Nterm] |
Two CpxR CDS, each N terminus attached to a moiety of IFP |
Characterize CpxR dimerization |
Bacteria |
| BBa_K1486010 |
CxpR & Split IFP1.4 [Nterm + Cterm] |
Two CpxR CDS, each attached to a moiety of IFP |
Characterize CpxR dimerization |
Bacteria |
| BBa_K1486011 |
CxpR & Split IFP1.4 [Cterm + Nterm] |
Two CpxR CDS, each attached to a moiety of IFP |
Characterize CpxR dimerization |
Bacteria |
| BBa_K1486012 |
CpxR + IFP[1] |
CpxR with the Nterm moiety of IFP attached at its C terminus |
Intermediate & control |
Bacteria |
| BBa_K1486013 |
CpxR + IFP[2] |
CpxR with the Cterm moiety of IFP attached at its C terminus |
Intermediate & control |
Bacteria |
| BBa_K1486014 |
IFP[1] + CpxR |
CpxR with the Nterm moiety of IFP attached at its N terminus |
Intermediate & control |
Bacteria |
| BBa_K1486015 |
IFP[2] + CpxR |
CpxR with the Cterm moiety of IFP attached at its N terminus |
Intermediate & control |
Bacteria |
| BBa_K1486016 |
fLuc[1] |
N terminus moiety of the firefly luciferase |
|
Bacteria |
| BBa_K1486017 |
fLuc[2] |
C terminus moiety of the firefly luciferase |
|
Bacteria |
| BBa_K1486018 |
PAra + fLuc[1] + fLuc[2] |
Split firefly luciferase under arabinose promoter |
Control |
Bacteria |
| BBa_K1486019 |
rLuc[1] |
C terminus moiety of the renilla luciferase |
|
Bacteria |
| BBa_K1486020 |
rLuc[2] |
N terminus moiety of the renilla luciferase |
|
Bacteria |
| BBa_K1486021 |
PAra + rLuc[1] + rLuc[2] |
Split renilla luciferase under arabinose promoter |
Control |
Bacteria |
| BBa_K1486022 |
rLuc |
Full renilla luciferase |
Control |
Bacteria |
| BBa_K1486023 |
Yeast sfGFP |
Superfolder GFP for yeast cells |
Reporter |
Yeast |
| BBa_K1486024 |
Kan |
Yeast kanamycin resistance gene |
Selection marker |
Yeast |
| BBa_K1486025 |
ADH1 terminator |
Terminator |
|
Yeast |
| BBa_K1486026 |
Yeast sfGFP + Kan |
Yeast sfGFP attached to the yeast kanamycin resistance gene |
Control the expression of pbs2 |
Yeast |
| BBa_K1486027 |
rLuc + Kan |
Renilla luciferase attached to the kanamycin resistance gene |
|
Yeast |
| BBa_K1486028 |
Yeast sfGFP[1] |
N terminal moiety of split yeast sfGFP |
|
Yeast |
| BBa_K1486029 |
sfGFP[1] + kan |
Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene |
|
Yeast |
| BBa_K1486030 |
rLuc[1] + kan |
Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene |
|
Yeast |
| BBa_K1486031 |
Ura |
CDS for Uracil (yeast selective purposes) |
Confer resistance to Uracil-deprived medium |
Yeast |
| BBa_K1486032 |
Yeast sfGFP + Ura |
Yeast sfGFP attached to the Uracil CDS |
Control the expression of hog1 |
Yeast |
| BBa_K1486033 |
rLuc + Ura |
Renilla luciferase attached to the Uracil CDS |
Control the expression of hog1 |
Yeast |
| BBa_K1486034 |
yeast sfGFP[2] |
C terminal moiety of split the yeast sfGFP |
|
Yeast |
| BBa_K1486035 |
yeast sfGFP[2] + Ura |
Cterm moiety of split yeast sfGFP attached to the Uracil CDS |
|
Yeast |
| BBa_K1486036 |
rLuc[2] + Ura |
Cterm moiety of split renilla luciferase attached to the Uracil CDS |
|
Yeast |
| BBa_K1486037 |
linker |
Attaches two proteins together |
|
Yeast |
| BBa_K1486038 |
sfGFP[1] |
N terminus moiety of split superfolder GFP |
|
Bacteria |
| BBa_K1486039 |
sfGFP[2] |
C terminus moiety of split superfolder GFP |
|
Bacteria |
| BBa_K1486040 |
sfGFP[1] + CpxR |
N terminus moiety of split sfGFP attached to CpxR |
|
Bacteria |
| BBa_K1486041 |
sfGFP[2] + CpxR |
C terminus moiety of split sfGFP attached to CpxR |
|
Bacteria |
| BBa_K1486042 |
LZip |
Monomer of leucine zipper TF |
|
Bacteria |
| BBa_K1486043 |
LZip + split rLuc |
Two Leucine Zipper monomers, each attached to a different split rLuc moiety |
Control for split rLuc assays |
Bacteria |
| BBa_K1486044 |
mut IFP[1] |
Biobrick-compatible IFP[1] |
|
Bacteria |
| BBa_K1486045 |
mut IFP[2] |
Biobrick-compatible IFP[2] |
|
Bacteria |
| BBa_K1486046 |
CpxR promoter FW |
CpxR binding-region in forward direction |
|
Bacteria |
| BBa_K1486047 |
CpxR promoter RV |
CpxR binding-region in reverse direction |
|
Bacteria |
| BBa_K1486048 |
CpxR reporter |
Calgary's CpxR reporter repaired (sequence was missing) |
To see when CpxR is active |
Bacteria |
| BBa_K1486049 |
CpxR promoter FW + RFP |
Reporter of CpxR |
Test the direction of the complete CpxR promoter |
Bacteria |
| BBa_K1486050 |
CpxR promoter RV + RFP |
Reporter of CpxR |
Test the direction of the complete CpxR promoter |
Bacteria |
| BBa_K1486053 |
Linker |
10 amino-acid linker |
Attach CheY/Z to split luciferases |
Bacteria |
| BBa_K1486054 |
CheY/CheZ rLuc |
CheY and CheZ, each attached to a moiety of split renilla luciferase |
Positive control for the split rLuc |
Bacteria |
| BBa_K1486055 |
CheY/CheZ fLuc |
CheY and CheZ, each attached to a moiety of split firefly luciferase |
Positive control for the split fLuc |
Bacteria |
| BBa_K1486056 |
CxpR & Split mut IFP1.4 [Cterm + Cterm] |
Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP |
Characterize CpxR dimerization |
Bacteria |
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