Team:Washington/Our Project

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Background

Our System

Method

Analysis of Test Protein Stability

Cloning in Escherichia coli

Preparation and Passaging of Saccharomyces cerevisiae

Relative Stability Analyzed via Flow Cytometry

Mutagenesis through Error Prone Polymerase Chain Reactions

Selecting Stable Variants through Fluorescence Activated Cell Sorting

Results

Protein stability analysis using Thermal and Guanadine-HCl melts

Protein stability analysis using Degron Constructs and Flow Cytometry

Mutagenesis Results and Mutant Variant Analysis

Future Plans

Examination of more proteins

Submitted Parts

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