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Team:BYU Provo/Notebook/Auxotrophy/febapr
From 2014.igem.org
BYU 2014 Notebook |
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Week of March 9th
March 12, 2014
--CB TR-- Researched possible kill switch options for N. multiformis. Focused on the environment of the bio-reactor to control spread of N. multiformis.
Originally we considered designing a trigger that is specific to the environment of the bioreactor that, when removed from the environment would express a gene that would kill the bacteria. However we realized that such a design would be problematic due to the high mutation rate of N. multiformis.
Week of March 16th
March 17, 2014
--CB TR-- Researched articles on the environment of waste water treatment plants and the possible genes we could knock out to prepare for our presentation on N.multiformis metabolism optimization.
Focused our efforts on a unique gene knock out that would make N. multiformis reliant on the environment of the bio-reactor to grow.
March 18, 2014
--CB TR-- Reviewed all of our literature findings so far. In doing so, identified the specific focuses for our group: inserting the denitrification genes into N. multiformis, making N. multiformis more resistant to pH changes, and making N. multiformis more resistant to heavy metals.
March 19, 2014
--CB TR-- Presented our ideas for improving the metabolism of N. multiformis and received feedback from the class on them. Confirmed plan to insert the denitrification pathway into N. multiformis. Decided to forego other original goals and instead insert genes that would break down antibiotics.
March 20, 2014
March 21, 2014
Week of March 23rd, 2014
March 24, 2014
--CB TR-- Continued researching auxtrophy ideas.
March 26, 2014
March 28, 2014
Week of March 27th
March 31, 2014
April 1, 2014
April 2, 2014
April 3, 2014
April 4, 2014
Week of April 12th, 2014
April 7, 2014
April 9, 2014
April 11, 2014
Week of April 19th
April 14, 2014
Week of April 26th
April 21, 2014
Our team prepared a semester final on our N.multiformis Serine Auxotrophy Process.
