Team:NCTU Formosa/Notebook/test5

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4/11 Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2) Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3) Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)

4/12 Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2) Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1) Cultivation of [mGFP] Ar (G3)

4/13 Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3) Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2) Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)

4/17 Transform of [PSB1K3] Kr (G1)

4/18 Cultivation of [PSB1K3] Kr (G1)

4/19 Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3) Mini-prep of [PSB1K3] Kr(G1)

4/20 Transform of [PSB1A3] Ar (G2) Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)

4/21 Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)

4/22 Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)

4/28 Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1) Cultivation of [PSB1C3] Cr (G1)

4/29 Mini-prep of [PSB1C3] Cr(G1) Mini-prep of [PSB1C3]Cr (G2) Transform of [B0032]Ar, and [BFP]Cr (G2) Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1) Cultivation of [B0032]Ar, and [BFP]Cr (G2)

4/30 Mini-prep of [PSB1A3] Ar (G1) Mini-prep of [B0032]Ar, and [BFP]Cr (G2) Transform of [B0032]Ar, and [BFP]Cr, [T7promoter]ArKr and [T7 polymerase]Cr (G2) Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Digestion of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2) Electrophoresis of plasmid and digested prouct of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)