Team:TU Delft-Leiden/WetLab/landmine/characterisation

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Landmine Module - Characterisation

As already mentioned, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2.

Assays

The different assays used to test our Land Mine BioBricks are:

  • Plate Reader

  • Microscopy

  • FACS

    • Plate Reader

    A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. The protocol followed for this module can be found at

    LINK TO PROTOCOLS

    Results - Plate Reader

    Microscopy

    FACS

    Fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry

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