| Name | Sequence (5'-3') | Theoretical Tm | Purpose | |
|---|
| iGEM10 | AGCTTTCGCTAAGGATGATTTCTGGA | 58.2C | Screening and sequencing |
| iGEM16 | GAGTGCCACCTGACGTCTAAGAAACC | 60.9C | For BioBrick sequencing, amplification by PCR, or junction PCR. Alternative to iGEM VF2 primer |
| iGEM25 | CCCTGATTCTGTGGATAACCGTATTACCG | 60.0C | For BioBrick sequencing, amplification by PCR, or junction PCR. Alternative to iGEM VR primer |
| iGEM1401 | GGGAAGCTTTCTTAGACGTCAGGTGGCACTTTTCGG | 66.3C | pBBR replicon |
| iGEM1402 | TTTGGATCCTATGCTGCTGGCTACCCTGTGGAAC | 66.5C | pBBR replicon |
\
| iGEM1403 | GTTCCACAGGGTAGCCAGCAGCATAGGATCCAAAGAGTGCCACCTGACGTCTAAGAAACC | 71.2C | primers for pSB prefix/suffix |
| iGEM1404 | CCGAAAAGTGCCACCTGACGTCTAAGAAAGCTTCCCCCTGATTCTGTGGATAACCGTATTACCG | 70.3 | primers for pSB prefix/suffix |
| iGEM1405 | GAGGTCGGACAGTATATTGAAACGTCAGGAGTCTAGATACCGAGGGACAGACTACCAACTCACA | | primers for gene cassette circularisation (attC-aeBlue-aacC1) |
| iGEM1406 | GTATCTAGACTCCTGACGTTTCAATATACTGTCCGACCTCTTATTAGGTGGCGGTACTTGGGTCG | | primers for gene cassette circularisation (attC-aeBlue-aacC1) |
| iGEM1407 | CTATATCTATGATCTCGCAGTCTCC | 53.8C | PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening |
|-
|iGEM1408 || GCCTTTTGCTCACATGTTCTTTC || 55.2C || PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening
|-
|iGEM1409 || GTTTTTTTGGATGGAGTGAAACGATGGCGATTG || 61.7C || Amplification of iGEM-IntI1 gBlock forward primer
|-
|iGEM1410 || TGACACCTTGCCCTTTTTTGCCG || 60.9C || Amplification of iGEM-IntI1 gBlock reverse primer
|-
|iGEM1411 || GTTTTTTTGGATGGAGTGAAACGATGGCGATTG || 61.7C || Gblock primers for IntI1 in pSAM-R
|-
|iGEM1412 || TGACACCTTGCCCTTTTTTG || 53.9C || Gblock primers for IntI1 in pSAM-R
|-
|iGEM1413 || GACATTGCCGTCACTGCGTC || 59.1C || Junction primers for IntI1 in pSAM-R
|-
|iGEM1414 || TGGTCCAGAACCTTGACCGAAC || 59.2C || Junction primers for IntI1 in pSAM-R
|-
|iGEM1415 || AACCGAGGATGCGAACCACTTC || 59.7C || Junction primers for IntI1 in pSAM-R
|-
|iGEM1416 || CCTTCAAACGTGCCGTAGAACAAGC || 60.4C || Junction primers for IntI1 in pSAM-R
|-
|iGEM1417 || AAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG || 67.6C || Linearisation of pSB1C3, containing full BioBrick prefix and suffix
|-
|iGEM1418 || AAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC || 66.9C || Linearisation of pSB1C3, containing full BioBrick prefix and suffix
|-
|iGEM1419 || TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGAC || 68.9C || Amplification of aeBlue gBlock. Forward primer.
|-
|iGEM1420 || GGGCTGCAGCGGCCGCTACTAGTATTATTAGGTGG || 67.4C || Amplification of aeBlue gBlock. Reverse primer.
|-
|iGEM1421 || GGGAATTCAAATCTAGAGACACCATCG || 57.1C || Amplification of LacI-Plac-AttI gBlock. Forward primer
|-
|iGEM1422 || CCTGCAGTTTACTAGTTTTGCCTAACTTTGTTTTAG || 59.4C || Amplification of LacI-Plac-AttI gBlock. Reverse primer
|-
|iGEM1423 || CAGGCTTTACACTTTATGCTTCCG || 56.3C || lacI-Plac-attI RHJ-F right hand junction forward primer (use with iGEM25)
|-
|iGEM1424 || AATGTAATTCAGCTCCGCCATC|| 55.5C || lacI-Plac-attI LHJ-R left hand junction reverse primer (use with iGEM16)
|-
|iGEM1425 || AAATCTAGATGGCGCGGCTTAACTCAGGTGTTAGGCTTAGGAGGCTCAAGTATGGGCAT || 70.7C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1426.
|-
|iGEM1426 || AAAACTAGTGGCGTCGGCTTGGACGAATTGTTAGGCTTAGGTGGCGGTACTTGGGTC || 71.4C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1425.
|-
|iGEM1427 || TTTTCTAGAGTTTGATGTTATGGAGCAGCAACG || 60.2C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1428. Alternative to iGEM1424.
|-
|iGEM1428 || TTTACTAGTGCAAAAAGGCAGCAATTATGAGCC || 60.9C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1427. Alternative to iGEM1426.
|-
|NVC71 || CTAAGAATCCATAGTCCAACTCC || 52.4C || aadB gene, rvs primer for junction screen
|-
|NVC92b || CACGCAAGACCTCAACCTTTTCC || 58.6C || aadB gene, rvs primer for junction screen
|-
|NVC158 || GATACCTTGTGCGGCTATGTCTG || 57.6C || pUS41/44, left of cloning site
|-
|NVC159 || GCCGCCTTGGGCCGGGTGATGTC || 69.2C || pUS41/44, right of cloning site
|}
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