Team:ETH Zurich/lab/biobrick/used1

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<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014

Contents

Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration

Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL

System considered

Modeling leakiness =

Results

Characterization of two-level crosstalk on the promoter

Systems considered

Modeling crosstalk

First-level crosstalk: LuxR binds to different HSL and activates the promoter

Second-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter

Combination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoter

Results

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