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7/31

Restriction Enzyme Digestion

Nakamura and Matsumoto

Sample/(µL)		EcoR1/(µL)	Xba1/(µL)	Spe1/(µL)	Pst1/(µL)	Buffer/(µL)		BSA/(µL)	MilliQ/(µL)	Total/(µL)
3/24 PCR(dddD) cloning	3.2	1	-	1	-	H	3	-	21.8	30
7/18 RBS30	18.5	1	-	1	-	H	3	-	6.5	30
7/18 RBS30 control	6.2	-	-	-	-	H	1	-	2.8	10