Team:Evry/Interlab Study/09-11-2014
From 2014.igem.org
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
- Transformation plate from the 10th September observation. There were 50 colonies for each constructions.
- PCR colony of 8 colonies for each construction following protocol table 1 and 2. https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
- PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Preparation of samples to sequencing. N° XX
- Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
Sep 11