The Sensor

1. Fluorescent Protein Analysis

From our analysis of possible fluorescent protein reporters, we selected yellow fluorescent protein (YFP) and red fluorescent protein (RFP)

2. Construction: Overlap PCR

We first ligated the T7 promoter and RFP into the pSB3K3 plasmid, to create the second plasmid part of our sensor

Notebook Entries

Week One Week Two Week Three Week Four Week Five