STREAM/3 June 2014

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June 3, 2014

MiniPreps of MACH Cells Expressing Fluorescence

Purpose: To isolate plasmid DNA from MACH cells

Protocol from GeneJet Plasmid Miniprep kit: http://www.thermoscientificbio.com/uploadedfiles/resources/k0502-product-information.pdf

TECAN

Purpose: To measure fluorescence from MACH Cells

Cell Cultures of MACH Cells for Fluorescence: 3 mL LB and 300 μL cell culture at 10:45 AM Time point 1: 12:45 PM Time point 2: 4:45 PM

To analyze fluorescence at given time points

In Row D: 50 μL cell culture (at time point 1)

Used the following parameters to observe the gain and brightness of the following fluorescent proteins: