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Team:NCTU Formosa/Safty
From 2014.igem.org
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In our lab, the incubator is maintaining the temperature at 37 degrees Celsius, which is the optical temperature of E.coli growing. | In our lab, the incubator is maintaining the temperature at 37 degrees Celsius, which is the optical temperature of E.coli growing. | ||
In this year, our team was authorized to work with an non-pathogenic bacterial strain, the Escherichia coli DH5. The strain ais regularly employed in research, industry and study. Biosafety Level 1 standard regulations were strictly followed while using these strains.</p> | In this year, our team was authorized to work with an non-pathogenic bacterial strain, the Escherichia coli DH5. The strain ais regularly employed in research, industry and study. Biosafety Level 1 standard regulations were strictly followed while using these strains.</p> | ||
| - | + | ||
| + | 2.Autoclave | ||
| + | <p>The autoclave is used for the safety issue. When sterilizing, the pressure and temperature inside reached high level, forming steam and superheat water. Cells will be killed and proteins will be destroyed or denatured. Thus, the safety is ensured. | ||
| + | </p><p>In our lab, every equipment may contact bacteria will be sterilized before and after use to guarantee the safety. | ||
| + | </p> | ||
</div></div> | </div></div> | ||
Revision as of 05:36, 2 September 2014
Researcher Safety
1.Everyone must wear lab coat, trousers, gloves, surgery masks and shoes when carrying out experiments.
2.Before the commencement of our project,our team instructors and advisers have guided us to go through every expiriment to make sure everybody is well trained and fit for this project.
3.Everybody has to sterilize his/her hands and all the equipment platforms with alcohol solution(70%) before and after each expiriment.
4.Emergency equipments are well prepared and all the researchers understand how to use these equipments and their exact position.
5.It is not allowed that researcher carries experiment alone.There must be at least two members to conduct the experiment.
6.Food and beverages are not allowed to appear in the lab.
Public safety
1.All collected lab waste are sterilized, packaged and executed.
2.E.coli used in our lab is less competitive than wild type, because they are unable to form biofilms and to thrive in the intestine.
3.Liquid waste that contains E.coli is disinfected by adding peroxides and cleaners before it is disposed.
Instruments in our lab
1.incubatorThe orbital shaker incubator is widely used in microbiology labs, cell biology labs, etc. It provides a stationary temperature to cultivate bacteria and cells. Some incubator also have functions to maintain the humility level. In our lab, the incubator is maintaining the temperature at 37 degrees Celsius, which is the optical temperature of E.coli growing. In this year, our team was authorized to work with an non-pathogenic bacterial strain, the Escherichia coli DH5. The strain ais regularly employed in research, industry and study. Biosafety Level 1 standard regulations were strictly followed while using these strains.
2.Autoclave
The autoclave is used for the safety issue. When sterilizing, the pressure and temperature inside reached high level, forming steam and superheat water. Cells will be killed and proteins will be destroyed or denatured. Thus, the safety is ensured.
In our lab, every equipment may contact bacteria will be sterilized before and after use to guarantee the safety.
