Team:Evry/Notebook/Sensing/PCBs/08-14-2014

From 2014.igem.org

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<u>Sensor construction HbpR/PhbpC/HbpC:</u>
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<li>Culturing DH5a (pHYB130 plasmid) in 3mL LB + 3µL Amp
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<li>Incubation at 37°C, 200rpm
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<u>Survival test on E.coli BL21:</u>
<u>Survival test on E.coli BL21:</u>

Revision as of 21:36, 1 September 2014

Picture


Survival test on E.coli BL21:
This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl

  1. Culturing of 200µL BL21 in 4mL of LB
  2. Incubation overnight at 37°C, 200rpm

Aug 14