Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014

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Revision as of 14:31, 29 August 2014

Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)

Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate) (LIEN PROTO).
After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.

Aug 23

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-	<u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 </b> </u> <br>	+	<u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21</b> </u> <br>

	Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)		Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)