"
Team:Evry/Interlab Study/08-22-2014
From 2014.igem.org
(Difference between revisions)
| Line 26: | Line 26: | ||
</a> | </a> | ||
</div> | </div> | ||
| - | <center>1% agarose gel of PCR products | + | <center>1% agarose gel of PCR products. Lane 1 to 8: BBa_K823012 PCR products and Lane 9: Purple 2-Log. ladder NEB</center> |
</div> | </div> | ||
</div> | </div> | ||
| Line 32: | Line 32: | ||
</div> | </div> | ||
| - | <br/> We expected to obtain a band between 300 and 400 bp. | + | <div class="center"> |
| + | <div class="thumb tnone"> | ||
| + | <div class="thumbinner" style="width:502px;"> | ||
| + | <a href="URL IMAGE" class="image"> | ||
| + | <img alt="IMAGE" src="URL IMAGE" width="500px;" class="thumbimage"/> | ||
| + | </a> | ||
| + | <div class="thumbcaption"> | ||
| + | <div class="magnify"> | ||
| + | <a href="URL IMAGE" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>1% agarose gel of PCR products. Lane 1 to 8: BBa_J23101 PCR products and Lane 9: Purple 2-Log. ladder NEB</center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <br/> We expected to obtain a band between 300 and 400 bp. So we decided to amplify the colony n°8. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation. | ||
| + | <br/> | ||
| + | |||
| + | |||
Revision as of 12:27, 29 August 2014
Contructions PSB1C3 with I20260, J23101-E1010 and K823012-E1010
Transformation plate observation:
- BBa_J23101: 11 isolated colonies
- BBa_K823012: 100-150 isolated colonies
A PCR was performed on 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
We expected to obtain a band between 300 and 400 bp. So we decided to amplify the colony n°8. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation.
Aug 22
