Team:ETH Zurich/labblog/20140825meet

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Revision as of 14:39, 28 August 2014

We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.

We have a Comsol simulation for this experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.

We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.

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	== Week 14 ==		== Week 14 ==