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Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
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| - | <u> <b> | + | <u> <b> Amplification of plasmids pBBR1MCS and phokHI-2 </b> </u> <br> |
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| + | Two plasmids pBBR1MCS and phokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology) | ||
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<img src="" alt="pBBR1MCS"/> | <img src="" alt="pBBR1MCS"/> | ||
<img src="" alt="phokH1-2"/> | <img src="" alt="phokH1-2"/> | ||
| - | + | </div> | |
| - | + | <br> | |
| - | + | Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate). After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. | |
Revision as of 11:51, 28 August 2014
Amplification of plasmids pBBR1MCS and phokHI-2
Two plasmids pBBR1MCS and phokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate). After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. Aug 23
