Revision as of 07:57, 28 August 2014

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Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very ustoeful organizational tool as well.

7/15

transformation

Name	Sample(µl)	Competent Cells(µl)	Total(µl)	Plate
BBa_J23101	2	20(2/23)	22	LB（CP）
BBA_B0032	2	20（2/23）	22	LB（CP）

7/16

transformation

Name	Sample(µl)	Competent Cells(µl)	Total(µl)	Plate
BBa_B0030	2	20(2/23)	22	LB（CP）
BBA_B0032	2	20（2/23）	22	LB（CP）

7/17

Colony PCR

Sample
7/16 BBa_B0030-(1)
7/16 BBa_B0030-(2)
7/16 BBa_B0030-(3)
7/16 BBa_B0030-(4)
7/16 BBa_B0030-(5)
7/16 BBa_B0030-(6)
7/16 BBa_B0032-(1)
7/16 BBa_B0032-(2)
7/16 BBa_B0032-(3)
7/16 BBa_B0032-(4)
7/16 BBa_B0032-(5)
7/16 BBa_B0032-(6)
NC

Liquid Culture

Sample	medium
7/16 BBa_B0030-(1)	Plusgrow medium(+CP)
7/16 BBa_B0030-(2)	Plusgrow medium(+CP)
7/16 BBa_B0030-(3)	Plusgrow medium(+CP)
7/16 BBa_B0030-(4)	Plusgrow medium(+CP)
7/16 BBa_B0030-(5)	Plusgrow medium(+CP)
7/16 BBa_B0030-(6)	Plusgrow medium(+CP)
7/16 BBa_B0032-(1)	Plusgrow medium(+CP)
7/16 BBa_B0032-(2)	Plusgrow medium(+CP)
7/16 BBa_B0032-(3)	Plusgrow medium(+CP)
7/16 BBa_B0032-(4)	Plusgrow medium(+CP)
7/16 BBa_B0032-(5)	Plusgrow medium(+CP)
7/16 BBa_B0032-(6)	Plusgrow medium(+CP)

7/18

Miniprep

DNA	Concentration(µg/ml)	260/280	260/230
7/16 BBa_B0030-(1)	229	1.96	1.40
7/16 BBa_B0030-(2)	209	1.95	1.50
7/16 BBa_B0030-(3)	108	2.12	2.12
7/16 BBa_B0030-(4)	186	-	-
7/16 BBa_B0030-(5)	174	2.02	1.56
7/16 BBa_B0030-(6)	158	2.01	1.68
7/16 BBa_B0032-(1)	179	2.00	2.06
7/16 BBa_B0032-(3)	221	1.90	1.37
7/16 BBa_B0032-(4)	225	1.89	1.37
7/16 BBa_B0032-(5)	270	1.98	1.63
7/16 BBa_B0032-(6)	194	1.89	1.33

7/28

Restriction Enzyme Digestion

7/18 RBS Sample	EcoR1	Xba1	Spe1	Pst1	Buffur	BSA	MilliQ	TOTAL
Sample1	0.3	0	0	0	1(H)	0	7.7	10
Sample2	0	0.3	0	0	1(M)	1	6.7	10
Sample3	0	0	0.3	0	1(M)	0	7.7	10
Sample4	0	0	0	0.3	1(H)	0	7.7	10
Control	0	0	0	0	1	0	8	10

@@ Line 193: / Line 193: @@
 <tr><td>7/16 BBa_B0032-(6)</td><td>194</td><td>1.89</td><td>1.33</td></tr>
 </table>
+<h2>7/28</h2>
+<h3>Restriction Enzyme Digestion</h3>
+<table class="note">
+<tr><th>7/18 RBS Sample</th><th>EcoR1</th><th>Xba1</th><th>Spe1</th><th>Pst1</th><th>Buffur</th><th>BSA</th><th>MilliQ</th><th>TOTAL</tr>
+<tr><td>Sample1</td><td>0.3</td><td>0</td><td>0</td><td>0</td><td>1(H)</td><td>0</td><td>7.7</td><td>10</td></tr>
+<tr><td>Sample2</td><td>0</td><td>0.3</td><td>0</td><td>0</td><td>1(M)</td><td>1</td><td>6.7</td><td>10</td></tr>
+<tr><td>Sample3</td><td>0</td><td>0</td><td>0.3</td><td>0</td><td>1(M)</td><td>0</td><td>7.7</td><td>10</td></tr>
+<tr><td>Sample4</td><td>0</td><td>0</td><td>0</td><td>0.3</td><td>1(H)</td><td>0</td><td>7.7</td><td>10</td></tr>
+<tr><td>Control</td><td>0</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td><td>8</td><td>10</td></tr>
+</table>

Team:Kyoto/Notebook

From 2014.igem.org

Revision as of 07:57, 28 August 2014

Notebook

7/15

transformation

7/16

transformation

7/17

Colony PCR

Liquid Culture

7/18

Miniprep

7/28

Restriction Enzyme Digestion