Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-18-2014
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Revision as of 17:51, 25 August 2014
Tests of antibiotics' stocks - Results(2)
Plates | LB Agar only | LB+Kan | LB+Erm (1:100) |
E.coli | X | 0 | 0 |
Launch of antibiotics's tests in liquid M9 1X For each tested antibiotic we made the following range of concentrations:
0 | 1:500 | 1:1000 | 1:50 000 | 1:100 000 |
/ | 0 | 1:500 | 1:1000 | 1:50 000 | 1:100 000 |
Medium+agar | 3mL | 3mL | 3mL | 3mL | 3mL |
Antibiotic | 0 | 6µL of stock | 3µL of stock | 6µL of stock diluted 1/100 | 3µL of stock diluted 1/100 |
Pre-culture | 300µL | 300µL | 300µL | 300µL | 300µL |
/ | Pseudo | Bl21 | DH5a | Top 10 | DH5a pyr+KanR | DH5a+CamR | Top 10+ErmR |
OD init | 0.02 | 0.795 | 0.873 | 0.693 | 0.408 | 0.687 | 0.319 |
For each tested antibiotic we made the same range of concentrations than in liquid. We also made these range two time to have a replicate and we added a controle plate without antibiotic on which we showed all our tested bacteria. Plates will be checked during three days. We made these same ranges of tested antibiotic's concentrations for three media:
- MB agar 1X
- MB agar 0.5X
- M9 agar 1X
/ | 0 | 1:500 | 1:1000 | 1:50 000 | 1:100 000 |
Medium+agar | 20mL | 20mL | 20mL | 20mL | 20mL |
Antibiotic | 0 | 40µL of stock | 20µL of stock | 40µL of stock diluted 1/100 | 20µL of stock diluted 1/100 |