Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-16-2014
From 2014.igem.org
(Difference between revisions)
Line 16: | Line 16: | ||
<li> Ampicilin | <li> Ampicilin | ||
<li> Tetracyclin | <li> Tetracyclin | ||
- | </ul | + | </ul> |
(The last one was the control of the growth of our bacteria without antibiotics) | (The last one was the control of the growth of our bacteria without antibiotics) | ||
We sowed 10µL of a liquid LB culture of Bl21, and we let them incubate overnight at 37°C.<br> | We sowed 10µL of a liquid LB culture of Bl21, and we let them incubate overnight at 37°C.<br> | ||
Line 32: | Line 32: | ||
<li>Top10 | <li>Top10 | ||
<li>DH5a | <li>DH5a | ||
- | </ul | + | </ul> |
<u>From plates:</u> | <u>From plates:</u> | ||
<ul> | <ul> | ||
Line 38: | Line 38: | ||
<li>Top10 transformed with pQexp | <li>Top10 transformed with pQexp | ||
<li>DH5a pyr tranformed with pMK2 | <li>DH5a pyr tranformed with pMK2 | ||
- | </ul | + | </ul> |
Bacteria tranformed with plasmid were cultivated with the corresponding antibiotic for the selection. | Bacteria tranformed with plasmid were cultivated with the corresponding antibiotic for the selection. | ||
For each medium we make a negative contrôle without bacteria. | For each medium we make a negative contrôle without bacteria. |
Revision as of 17:38, 25 August 2014
Tests of antibiotics' stocks
Six plates of LB agar were made.
Five of them contained one of those antibiotics in the dilution 1:1000:
- Chloramphénicol
- Kanamycin
- Erythromycin
- Ampicilin
- Tetracyclin
Survivability tests
Two plates of MB 1X and M9 1X were made and divised in two parts. Each plate was sowed with 5µL of a liquid MB 1X culture of Pseudovibrio denitrificans(dated 12th August) in a part and with 5µL of a liquid M9 1X culture Pseudovibrio denitrificans (dated 12th August) in the other part.
Pre-cultures
Cultures in MB 1X and M9 1X of Pseudovibrio were passed by a 1/10 dilution and let incubate overnight at 30°C. New cultures of different E.Coli were also started from glycerol or plate and let incubated overnight at 37°C.
From glycerol stocks:
- Bl21
- Top10
- DH5a
- DH5a tranformed with pCB1C3
- Top10 transformed with pQexp
- DH5a pyr tranformed with pMK2