Team:Evry/Notebook/Sensing/PCBs/08-20-2014

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(Difference between revisions)
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<u>Sensor construction hbpR2/PhbpR1</u>
<u>Sensor construction hbpR2/PhbpR1</u>
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<br/>We received sequencing reads: that match perfectly to the registry sequence.
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<br>We received sequencing reads: that match perfectly to the registry sequence.
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<br/>26DJ54
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<br>26DJ54
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<br/>AATAGGCGTTATCACGAGGCAGAANTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCCCTGG
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<br>AATAGGCGTTATCACGAGGCAGAANTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCCCTGG
GTGACATGCGTGATTTGGCCGCCACGCGGATCGCGCTCGAGAGCGAAGCGTTACGCCAAAGCGTGCTGAATGGTGACGCTGAATGGGAGGCGCGGATCGTCAGTTC
GTGACATGCGTGATTTGGCCGCCACGCGGATCGCGCTCGAGAGCGAAGCGTTACGCCAAAGCGTGCTGAATGGTGACGCTGAATGGGAGGCGCGGATCGTCAGTTC
GTTTCACCGACTGTCATTGATTGAAGAGCCCACGATGCGGGATCCGGCTCGCTGGTTTAATGAGTGGGAGCCAGTCAACCGCGGTTTTCACGAAGCTCTTATCTCT
GTTTCACCGACTGTCATTGATTGAAGAGCCCACGATGCGGGATCCGGCTCGCTGGTTTAATGAGTGGGAGCCAGTCAACCGCGGTTTTCACGAAGCTCTTATCTCT
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GGCTGCGGCGAGCGGTATCA
GGCTGCGGCGAGCGGTATCA
GCTCACTCAGGG
GCTCACTCAGGG
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<br/>
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<br>
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<br/>26DJ55
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<br>26DJ55
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<br/>CGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGC
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<br>CGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGC
AGCGGCCGCTACTAGTAAGCAGTCAATCGGCGGTAGCGCTCCATATGCACATACAGGATGGACAGGAACCGCCGGATCCAGACGGACGAACAGGCAGAGATAAGAG
AGCGGCCGCTACTAGTAAGCAGTCAATCGGCGGTAGCGCTCCATATGCACATACAGGATGGACAGGAACCGCCGGATCCAGACGGACGAACAGGCAGAGATAAGAG
CTTCGTGAAAACCGCGGTTGACTGGCTCCCACTCATTAAACCAGCGAGCCGGATCCCGCATCGTGGGCTCTTCAATCAATGACAGTCGGTGAAACGAACTGACGAT
CTTCGTGAAAACCGCGGTTGACTGGCTCCCACTCATTAAACCAGCGAGCCGGATCCCGCATCGTGGGCTCTTCAATCAATGACAGTCGGTGAAACGAACTGACGAT
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TGATAATAATGGTTTCTTAGA
TGATAATAATGGTTTCTTAGA
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<br/>
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<br>
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<br/><u>Sensor construction hbpR/PhbpC</u>
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<br><u>Sensor construction hbpR/PhbpC</u>
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<br/>The amplification by PCR was performed another time to amplify BBa_B0015 with overhangs. Protocol was the same as 08/19/2014. To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. A really slight band was visible between 200 and 300 bp. It was to weak to obtain a picture with the camera. According to the ladder scale, the band correspond to 20-30 ng, so approximately 2-3 ng/µl of DNA for the PCR product.
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<br>The amplification by PCR was performed another time to amplify BBa_B0015 with overhangs. Protocol was the same as 08/19/2014. To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. A really slight band was visible between 200 and 300 bp. It was to weak to obtain a picture with the camera. According to the ladder scale, the band correspond to 20-30 ng, so approximately 2-3 ng/µl of DNA for the PCR product.

Revision as of 10:26, 25 August 2014

Picture

Sensor construction hbpR2/PhbpR1
We received sequencing reads: that match perfectly to the registry sequence.
26DJ54
AATAGGCGTTATCACGAGGCAGAANTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCCCTGG GTGACATGCGTGATTTGGCCGCCACGCGGATCGCGCTCGAGAGCGAAGCGTTACGCCAAAGCGTGCTGAATGGTGACGCTGAATGGGAGGCGCGGATCGTCAGTTC GTTTCACCGACTGTCATTGATTGAAGAGCCCACGATGCGGGATCCGGCTCGCTGGTTTAATGAGTGGGAGCCAGTCAACCGCGGTTTTCACGAAGCTCTTATCTCT GCCTGTTCGTCCGTCTGGATCCGGCGGTTCCTGTCCATCCTGTATGTGCATATGGAGCGCTACCGCCGATTGACTGCTTACTAGTAGCGGCCGCTGCAGTCCGGCA AAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCA GCTCACTCAGGG

26DJ55
CGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGC AGCGGCCGCTACTAGTAAGCAGTCAATCGGCGGTAGCGCTCCATATGCACATACAGGATGGACAGGAACCGCCGGATCCAGACGGACGAACAGGCAGAGATAAGAG CTTCGTGAAAACCGCGGTTGACTGGCTCCCACTCATTAAACCAGCGAGCCGGATCCCGCATCGTGGGCTCTTCAATCAATGACAGTCGGTGAAACGAACTGACGAT CCGCGCCTCCCATTCAGCGTCACCATTCAGCACGCTTTGGCGTAACGCTTCGCTCTCGAGCGCGATCCGCGTGGCGGCCAAATCACGCATGTCACCCAGGGACATC TCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCA TGATAATAATGGTTTCTTAGA

Sensor construction hbpR/PhbpC
The amplification by PCR was performed another time to amplify BBa_B0015 with overhangs. Protocol was the same as 08/19/2014. To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. A really slight band was visible between 200 and 300 bp. It was to weak to obtain a picture with the camera. According to the ladder scale, the band correspond to 20-30 ng, so approximately 2-3 ng/µl of DNA for the PCR product.

Aug 20

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