Team:Evry/Notebook/Sensing/PCBs/08-06-2014

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<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
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<u><h4>Sensor construction HbpR/PhbpC:</h4></u>
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<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
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<li> Culturing of DH5a which contains pHYB109 plasmid with HbpR-PhbpC sequence on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp)  
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<li> Incubation overnight at 37°C
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Revision as of 10:07, 25 August 2014

Picture

Sensor construction HbpR/PhbpC:

  1. Culturing of DH5a which contains pHYB109 plasmid with HbpR-PhbpC sequence on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp)
  2. Incubation overnight at 37°C

Aug 6

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