Team:Evry/Notebook/Sensing/08-15-2014

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Revision as of 22:38, 24 August 2014

Week 7

Sensor construction HbpR/PhbpC/HbpC:

Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL

Survival test on E.coli BL21:

 A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
  1. Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
    image not found
  2. 300µL of E.coli BL21 was added in each eppendorf tube.
    We had 2 control tubes:
    • A negative control: 2,7mL of M9 medium + 300µL of compound
    • A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
  3. Incubation of tubes at 37°C overnight, 200rpm

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