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Team:UNIK Copenhagen/Logbook
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Revision as of 12:52, 22 August 2014
17th of July - amplification of pETDuet-1 (Q)
We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.
23rd of July (Q)
We made three liquid cultures each from a single colony on the plate.
24th of July(Q)
Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:
| Colony: | ng/µl | 260/280 | 260/230 |
| 1 | 30.3 | 1.96 | 2.34 |
| 2 | 63.3 | 1.82 | 1.89 |
| 3 | 32.1 | 1.82 | 1.68 |
On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.
20st of August
Ecloni was transformed with pUC57 containing sequences for either Estradiol light chain (EL) or Estradiol heavy chain (EH). The same was done for Progesterone light chain (PL) and heavy chain (PH). We also ran a PCR on pUC57, EL, EH, PL and PH.
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