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Team:UNIK Copenhagen/Logbook
From 2014.igem.org
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We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.<br><br> | We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.<br><br> | ||
<b>23rd of July (Q)</b><br>We made three liquid cultures each from a single colony on the plate.<br><br> | <b>23rd of July (Q)</b><br>We made three liquid cultures each from a single colony on the plate.<br><br> | ||
| - | <b>24th of July(Q)</b><br>Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:<br | + | <b>24th of July(Q)</b><br>Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:<br> |
<table><tr> | <table><tr> | ||
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<td>1.68</td> | <td>1.68</td> | ||
</tr></table> | </tr></table> | ||
| - | < | + | <br> |
| + | On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.</p> | ||
</div> | </div> | ||
<div class="right_page"> | <div class="right_page"> | ||
Revision as of 12:39, 22 August 2014
17th of July - amplification of pETDuet-1 (Q)
We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.
23rd of July (Q)
We made three liquid cultures each from a single colony on the plate.
24th of July(Q)
Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:
| Colony: | ng/µl | 260/280 | 260/230 |
| 1 | 30.3 | 1.96 | 2.34 |
| 2 | 63.3 | 1.82 | 1.89 |
| 3 | 32.1 | 1.82 | 1.68 |
On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.
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