"
Team:UNIK Copenhagen/Logbook
From 2014.igem.org
| Line 195: | Line 195: | ||
<td>1.68</td> | <td>1.68</td> | ||
</tr></table> | </tr></table> | ||
| + | <p>On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.</p> | ||
</div> | </div> | ||
<div class="right_page"> | <div class="right_page"> | ||
Revision as of 12:38, 22 August 2014
17th of July - amplification of pETDuet-1 (Q)
We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.
23rd of July (Q)
We made three liquid cultures each from a single colony on the plate.
24th of July(Q)
Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:
| Colony: | ng/µl | 260/280 | 260/230 |
| 1 | 30.3 | 1.96 | 2.34 |
| 2 | 63.3 | 1.82 | 1.89 |
| 3 | 32.1 | 1.82 | 1.68 |
On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.
Content1 (right).
Content2 (left)
Content2 (right)
Content3 (left)
Content3 (right)
Content4 (left)
Content4 (right)
Content5 (left)
Content5 (right)
Content6 (left)
Content6 (right)
Content7 (left)
Content7 (right)
Content8 (left)
Content8 (right)
Content9 (left)
Content9 (right)
Content10 (left)
Content10 (right)
