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Team:Warwick/Interlab
From 2014.igem.org
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The Interlab study is a voluntary project, new this year, which exists separate to each team's core work, and is an opportunity for iGEM HQ to understand how much variability exists between the practices and measurement methodologies of teams from across the globe. We decided to participate not only to support this objective, but also out of interest and as a means of diversification; the greater the number of strands along which we work, the less damaging any particular failure would be. It is suggested as a pre-summer activity but we only began in late July so it necessarily ran parallel with our principal work. | The Interlab study is a voluntary project, new this year, which exists separate to each team's core work, and is an opportunity for iGEM HQ to understand how much variability exists between the practices and measurement methodologies of teams from across the globe. We decided to participate not only to support this objective, but also out of interest and as a means of diversification; the greater the number of strands along which we work, the less damaging any particular failure would be. It is suggested as a pre-summer activity but we only began in late July so it necessarily ran parallel with our principal work. | ||
| - | The brief was thus: construct | + | The brief was thus: construct three given devices in plasmid format, all of which express green fluorescent protein (GFP), transfect them into E.coli and finally measure the fluorescence of these bacteria, using whatever technique you deem reasonable. |
Revision as of 14:17, 20 August 2014
WAQ'S WORK
We are taking part in the interlab study, where we will obtain and measure fluorescence data from three specific genetic devices expressing Green fluorescent protein (GFP) in E. coli . To view how we are going to do this experimentally and obtained results, click on the buttons below.
DAY ONE
Transformation of E. coli with devices following protocols found here (link to Notebook section)
DAY TWO
Transformations worked, going to regrow culture and then digest, ligate!
DAN'S WORK
The Interlab study is a voluntary project, new this year, which exists separate to each team's core work, and is an opportunity for iGEM HQ to understand how much variability exists between the practices and measurement methodologies of teams from across the globe. We decided to participate not only to support this objective, but also out of interest and as a means of diversification; the greater the number of strands along which we work, the less damaging any particular failure would be. It is suggested as a pre-summer activity but we only began in late July so it necessarily ran parallel with our principal work.
The brief was thus: construct three given devices in plasmid format, all of which express green fluorescent protein (GFP), transfect them into E.coli and finally measure the fluorescence of these bacteria, using whatever technique you deem reasonable.
