Revision as of 18:32, 19 August 2014

Tuesday, August 19, 2014

Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them.

We are sending the gRNA from the third batch and some other reporter vectors for sequencing.

Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.

Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.

~~Orientation! School is starting next week! (25/8) >.<~~

Today's tasks:
Miniprep ctxA, ctxB. Test digest and ran gel for ctxA, ctxB. Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb). Prepared for sequencing: diluted to the correct volume and concentration. Purified RBS (digested overnight yesterday). Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).

RBS-Carb Gel was good.

Description of image from today

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-       <br> Rishika sitting on the table.
+       <br> RBS-Carb Gel was good.
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