From 2014.igem.org
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| | <h3>Caffeine Project!</h3> | | <h3>Caffeine Project!</h3> |
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| | + | The 2012 UT Austin iGEM team isolated and refactored a gene cluster from Pseudomonas putida CBB5 that codes for a demethylation enzyme pathway to convert caffeine to xanthine, then cloned the plasmid into a xanthine biosynthesis knockout strain to “addict” the strain to caffeine. The strain was growth-limited by the amount of xanthine produced by the pathway, and the team was able to measure the amount of methylxanthines in a common beverage, such as caffeine in coffee, based on the amount of growth of the strain supplemented with the beverage.</p> |
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| | + | This year, our team seeks to better characterize the pathway and improve the measurement system. A beverage like coffee inherently contains various methylxanthines that can all feed into the enzyme pathway and allow growth. By creating a series of combinatorial knockout plasmids based on the refactored gene cluster, we should be able to more exactly measure amounts of methylxanthines specifically, as opposed to collectively as before, in a beverage. Additionally, by using standards, the plasmids will help to understand whether the pathway has a specific order, which is currently not defined. |
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Revision as of 21:09, 15 August 2014
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Welcome!
University of Texas at Austin
Scroll down to find more about our project!
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What is your project about?
Here you can explain your project in a short sentence or paragraph!
For example: Our team designed a biosensor to detect a certain chemical in water
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Photocage Project!
[Project Description Goes Here]
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Kit Project!
[Project Description Goes Here]
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Caffeine Project!
The 2012 UT Austin iGEM team isolated and refactored a gene cluster from Pseudomonas putida CBB5 that codes for a demethylation enzyme pathway to convert caffeine to xanthine, then cloned the plasmid into a xanthine biosynthesis knockout strain to “addict” the strain to caffeine. The strain was growth-limited by the amount of xanthine produced by the pathway, and the team was able to measure the amount of methylxanthines in a common beverage, such as caffeine in coffee, based on the amount of growth of the strain supplemented with the beverage.
This year, our team seeks to better characterize the pathway and improve the measurement system. A beverage like coffee inherently contains various methylxanthines that can all feed into the enzyme pathway and allow growth. By creating a series of combinatorial knockout plasmids based on the refactored gene cluster, we should be able to more exactly measure amounts of methylxanthines specifically, as opposed to collectively as before, in a beverage. Additionally, by using standards, the plasmids will help to understand whether the pathway has a specific order, which is currently not defined.
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Here you can place the logos of your sponsors or other links!
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