Team:Stony Brook

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Stony_Brook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/5/51/Stony_brook_igem_logo.png" alt= "iGEM logo" name="iGEM_logo" border="0px" id="iGEM_logo" /></a>
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<div id="header"><a href="https://2014.igem.org/Team:Stony_Brook"><img src="https://static.igem.org/mediawiki/2014/1/11/Stony_brook_header.png" alt="Stony Brook iGEM" border="0px"" /></a></div>
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    <div id="navigation"> <a href="https://2014.igem.org/Team:Stony_Brook"><img src="https://static.igem.org/mediawiki/2014/b/b8/Stony_Brook_NavIcon.Home.png"  border="0px" /></a>
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<p>Home</p>
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  <a href="https://2014.igem.org/Team:Stony_Brook/Team"><img src="https://static.igem.org/mediawiki/2014/4/4a/Stony_Brook_NavIcon.Team.png"  border="0px" /></a>
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<p>Team</p>
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  <a href="https://2014.igem.org/Team:Stony_Brook/Project"><img src="https://static.igem.org/mediawiki/2014/a/ab/Stony_Brook_NavIcon.Project.png" border="0px" /></a>
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<p>Project</p>
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<p>Notebook</p>
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      <p>Attributions</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Stony_Brook">Home</a> </li>
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  <li><a href="https://2014.igem.org/Team:Stony_Brook">page</a></li>
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<li><a href="https://2014.igem.org/Team:Stony_Brook/Team">Team</a> </li>
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  <li><a href="https://2014.igem.org/Talk:Team:Stony_Brook">discussion</a></li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Stony_Brook">Official Team Profile</a> </li>
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  <li><a href="https://2014.igem.org/wiki/index.php?title=Team:Stony_Brook&amp;action=edit">view source</a></li>
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<li><a href="https://2014.igem.org/Team:Stony_Brook/Project">Project</a> </li>
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  <li><a href="https://2014.igem.org/wiki/index.php?title=Team:Stony_Brook&amp;action=history">history</a></li>
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<li><a href="https://2014.igem.org/Team:Stony_Brook/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:Stony_Brook/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:Stony_Brook/Safety">Safety</a> </li>
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<h1> Project Description </h1>
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<p> The threat of antibiotic resistance has been a concern since the discovery of penicillin.  As antibiotics continue to be used to treat disease, pathogenic bacteria continue to develop resistance, eventually becoming irresponsive to multiple classes of antibiotics. This is a major problem for areas that must maintain sterile environments, such as hospitals or other health-care settings. Hospital-acquired infections (HAI) are already an existing issue that poses risks to people receiving or recovering from treatment. Gram-negative bacteria in particular are known to cause more HAIs as they are more resistant to antibiotics than gram-positive bacteria. Irresponsible use of antibiotics has only exacerbated this issue. Recent reports from the World Health Organization confirm that antibiotic resistance is no longer something to worry about in the future, but a current, global health threat. </p>
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<p> Alternatives to modern antibiotics, such as antimicrobial peptides, are now being researched. Antimicrobial peptides, or AMPs, are peptides produced innately by the immune systems of certain organisms to fight off infection. Because they kill microorganisms through different mechanisms, they have the potential to be effective against antibiotic resistant bacteria. One such AMP is melittin, produced by honey bees and used in their venom. Melittin kills cells by forming pores in the cell membrane, eventually destabilizing the cell and causing cell lysis. Because melittin targets a conserved area of cells, bacteria cannot develop resistance to it as easily as different types of antibiotics. </p>
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<p>There are a few wiki requirements teams must follow:</p>
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<p> Our project this summer is to transform E. coli with the melittin gene in honey bees, so that the E. coli may express the melittin peptide, thereby developing a means to mass-produce melittin as an alternative to today's antibiotics. We hope to optimize the production of melittin in E. coli, as well as to test the bioactivity of our produced melittin in comparison to its naturally-occurring counterpart. </p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Revision as of 15:52, 14 August 2014


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Project Description

The threat of antibiotic resistance has been a concern since the discovery of penicillin. As antibiotics continue to be used to treat disease, pathogenic bacteria continue to develop resistance, eventually becoming irresponsive to multiple classes of antibiotics. This is a major problem for areas that must maintain sterile environments, such as hospitals or other health-care settings. Hospital-acquired infections (HAI) are already an existing issue that poses risks to people receiving or recovering from treatment. Gram-negative bacteria in particular are known to cause more HAIs as they are more resistant to antibiotics than gram-positive bacteria. Irresponsible use of antibiotics has only exacerbated this issue. Recent reports from the World Health Organization confirm that antibiotic resistance is no longer something to worry about in the future, but a current, global health threat.

Alternatives to modern antibiotics, such as antimicrobial peptides, are now being researched. Antimicrobial peptides, or AMPs, are peptides produced innately by the immune systems of certain organisms to fight off infection. Because they kill microorganisms through different mechanisms, they have the potential to be effective against antibiotic resistant bacteria. One such AMP is melittin, produced by honey bees and used in their venom. Melittin kills cells by forming pores in the cell membrane, eventually destabilizing the cell and causing cell lysis. Because melittin targets a conserved area of cells, bacteria cannot develop resistance to it as easily as different types of antibiotics.

Our project this summer is to transform E. coli with the melittin gene in honey bees, so that the E. coli may express the melittin peptide, thereby developing a means to mass-produce melittin as an alternative to today's antibiotics. We hope to optimize the production of melittin in E. coli, as well as to test the bioactivity of our produced melittin in comparison to its naturally-occurring counterpart.

Retrieved from "http://2014.igem.org/Team:Stony_Brook"