Team:StanfordBrownSpelman/Cellulose Acetate
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Revision as of 22:24, 11 August 2014
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<a href="cellulose_acetate.html">Cellulose Acetate</a></h3>
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The goal of this subproject is to engineer Gluconacetobacter hansenii, which produces large quantities of bacterial cellulose (BC), to use the acetylation machinery found in the wrinkly spreader isolate SBW25 of Pseudomonas fluorescens to produce bacterial cellulose acetate (BCOAc) towards its ultimate application as the foundation of a fully biological UAV. Industrially-produced cellulose acetate has many uses as a synthetic fiber and has advantages over pure BC in terms of material properties. However, its production presents some issues in that it requires harsh chemical processes, making the biological manufacturing method highly valuable. Using directed evolution, we plan to select for those organisms which produce the polymer with highest acetate content. In addition, we seek to create a fusion protein which will have the capacity to both cross-link BCOAc polymers (improving material properties) and allow the modular addition of any cell (e.g. a biosensor). This will be accomplished through the expression of a biotinylated membrane protein, through the protein’s streptavidin domain, making the UAV highly programmable.
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The goal of this subproject is to engineer Gluconacetobacter hansenii, which produces large quantities of bacterial cellulose (BC), to use the acetylation machinery found in the wrinkly spreader isolate SBW25 of Pseudomonas fluorescens to produce bacterial cellulose acetate (BCOAc) towards its ultimate application as the foundation of a fully biological UAV. Industrially-produced cellulose acetate has many uses as a synthetic fiber and has advantages over pure BC in terms of material properties. However, its production presents some issues in that it requires harsh chemical processes, making the biological manufacturing method highly valuable. Using directed evolution, we plan to select for those organisms which produce the polymer with highest acetate content. In addition, we seek to create a fusion protein which will have the capacity to both cross-link BCOAc polymers (improving material properties) and allow the modular addition of any cell (e.g. a biosensor). This will be accomplished through the expression of a biotinylated membrane protein, through the protein’s streptavidin domain, making the UAV highly programmable.
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<center>Results
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Cras venenatis lorem et odio sodales, vitae aliquet nunc ultrices. Nunc lacinia nulla urna, sed aliquam nisl fermentum sed. Phasellus vel pellentesque tortor, in tincidunt metus. Aliquam ac laoreet risus. Fusce venenatis, justo id luctus dictum, turpis libero tincidunt mauris, sit amet tempor lectus tortor ut ante. Pellentesque egestas felis et est venenatis, eget lobortis dui adipiscing. Suspendisse volutpat sem eu ornare tincidunt. Mauris pharetra sed justo vitae sodales. Nulla in sodales tortor, placerat tempor dui.
<center>Methods & Safety
Fusce venenatis, justo id luctus dictum, turpis libero tincidunt mauris, sit amet tempor lectus tortor ut ante. Pellentesque egestas felis et est venenatis, eget lobortis dui adipiscing. Suspendisse volutpat sem eu ornare tincidunt. Mauris pharetra sed justo vitae sodales. Nulla in sodales tortor, placerat tempor dui.
<a href="work/PUT-PDF-REFERENCE-HEREpdf"><img src="images/download.png"></a><a href="work/PUT-PDF-REFERENCE-HEREpdf">Click here to download our project journal, which details our design and engineering process and included descriptions of the protocols we developed and used.</a>
<center>Links & References
Suspendisse volutpat sem eu ornare tincidunt. Mauris pharetra sed justo vitae sodales. Nulla in sodales tortor, placerat tempor dui.
<a href="work/PUT-PDF-REFERENCE-HEREpdf">● Have a link or reference? Put it here!</a>
<a href="work/PUT-PDF-REFERENCE-HEREpdf">● Link, acknowledgement, or reference 2</a>
<a href="work/PUT-PDF-REFERENCE-HEREpdf">● Additional links, acknowledgements, and references</a>
<center>Additional Information
Try to avoid having any additional information here. We're trying to keep our site organized, clean, and compelling!
Reach out: <a class="links" href="mailto:sbsigem2014@googlegroups.com">sbsigem@googlegroups.com</a>
<a class="links" href="pdfs/Stanford-Brown-Spelman_Past_And_Present_Projects.pdf" target="_blank">View our Complete Project List</a>
Reach out: <a class="links" href="mailto:sbsigem2014@googlegroups.com">sbsigem@googlegroups.com</a>
<a class="links" href="pdfs/Stanford-Brown-Spelman_Past_And_Present_Projects.pdf" target="_blank">View our Complete Project List</a>