Team:UT-Dallas/Notebook/8-11

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<h1 class="firstHeading" align="center">Sunday, August 10, 2014</h1>
<h1 class="firstHeading" align="center">Sunday, August 10, 2014</h1>
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<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-11&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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   <p> Purified plasmid DNA from potential Reporter clones for later testing. </p>
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   <p> We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.</p>
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  <p> Test purified plasmid DNA for correct insert. </P>
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       <th><font size="3">Today's tasks:</font>
       <th><font size="3">Today's tasks:</font>
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<p>Miniprep</p>
 
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<p>Inoculated more clones</p>
 
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<LI> Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
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<LI> Miniprep tcpS clones
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         <LI> Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.  
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<LI> Test digest Reporter plasmids for ctxA, tcpJ, tcpP, and tcpS
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         <LI> Streak M13.
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<LI> Ligate + transform chromoproteins [P+RBS+chromo]
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<LI> Set up overnight ligation for tcpD, toxT.
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       <br> Preparing cells for plasmid purification.
       <br> Preparing cells for plasmid purification.
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Revision as of 18:12, 11 August 2014

Sunday, August 10, 2014

Click here to edit the page

We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.
Today's tasks:
  • Miniprep tcpS clones
  • Test digest Reporter plasmids for ctxA, tcpJ, tcpP, and tcpS
  • Streak M13.
  • Ligate + transform chromoproteins [P+RBS+chromo]
  • Set up overnight ligation for tcpD, toxT.


Preparing cells for plasmid purification.


Description of image from today

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