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Team:NTNU Trondheim/Protocols
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Revision as of 13:53, 7 August 2014
Team:NTNU Trondheim/Protocols
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Team:NTNU_Trondheim/Protocols
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Media
- Liquid Broth (LB)
- Super Optimal Broth (S.O.C.)
- yB medium
- Synechocystis medium
-
Techniques
- Gibson assembly
- DNA isolation and cleaning
- DNA digestion
- PCR
- NanoDrop
- 3A assembly
- Ligation
- Transformation (Escherichia coli)
- Transformation (Synechocystis sp. PCC 6803)
- Gel electrophoresis
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Plasmids
- Right flank
- Left flank
- Kanamycin
- Lac inducible promoter
- Glucose oxidase
-
Organisms
- Escherichia coli DH5α
- Synechocystis sp. PCC 6803
-
Calculations
- Transformation efficiency
- Enzyme amount
- DNA concentration
Protocols
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Liquid Broth (LB)
Recipe (LB)
Antibiotic additions
Ingredients:
- Tryptone (10g)
- NaCl (10g)
- Yeast Extract (5g)
- Fill with 1 L of distilled / filtered H2O.
- Autoclave at 121 °C for 20 minutes.
- Add antibiotics if needed, after the medium has cooled down.
Week 19
(10/21 - 10/28)
Super Optimal Broth (S.O.C.)
Recipe
show technical details
{{{tech}}}
Made LB plates with ampicillin and ampicillin + kanamycin.
