Team:Paris Saclay/Notebook/July/31

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(Transformation in DH5α)
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''by Fabio''
''by Fabio''
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We transformed in DH5α the Ligation's result made [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System yesterday] ('''BBa_J61051''' and '''BBa_K228001''').
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We transformed in DH5α the Ligation's result made [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System yesterday] ('''BBa_J61051''' with '''BBa_K228001''').
'''Samples:'''
'''Samples:'''

Revision as of 17:56, 6 August 2014

Contents

Thursday 31st July

Lab work

C - Salicylate Inducible Suppressing System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 with BBa_K228001).

Samples:

  1. Sample 2 μl
  2. Sample 1 μl
  3. DH5α Control

Results on 1st August morning:

  1. Success, grown very well (+- 100 colonies)
  2. Success, grown well (+- 50 colonies)
  3. Success, nothing has grown

Protocol

D - Lemon scent

by Romain

2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.

Human Pratices

Reunion, Handled topics:

  • Organisation of the Meeting with french teams
  • Great discussion about Human Practices
  • Discussion about the logo

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Romain, Sean and Terry.

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