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Team:Paris Saclay/Protocols/PCR clean-up
From 2014.igem.org
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Collect your DNA in an eppendorf 1,5ml. | Collect your DNA in an eppendorf 1,5ml. | ||
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Revision as of 09:36, 4 August 2014
PCR clean-up
After the PCR, pool your PCR result in an eppendorf 1,5ml. Add 200µl NTI.
Centrifuge at 11000g, 30 secondes RT.
Discard the supernatant, wash first time with 700µl NT3.
Centrifuge at 11000g, 30 secondes RT.
Discard the supernatant, wash a second time with 700µl NT3.
Centrifuge at 11000g, 30 secondes RT.
Centrifuge the column empty.
Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT.
Collect your DNA in an eppendorf 1,5ml.
