Team:Paris Saclay/Notebook/August/1
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=Friday 1st August= | =Friday 1st August= | ||
==Lab work== | ==Lab work== | ||
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+ | ===A - The frame coli Odor free=== | ||
+ | ====Stria on LB dishes==== | ||
+ | ''by Romain'' | ||
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+ | Strains used: MG1655 and MG1655Z1, striated on 4 LB dishes per strain. | ||
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===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
====Liquid Culture==== | ====Liquid Culture==== |
Revision as of 09:36, 4 August 2014
Contents |
Friday 1st August
Lab work
A - The frame coli Odor free
Stria on LB dishes
by Romain
Strains used: MG1655 and MG1655Z1, striated on 4 LB dishes per strain.
C - Salicylate Inducible Suppressing System
Liquid Culture
by Fabio
8 liquid cultures with 3ml of LB and 3µl of amp (at 10am - 8°C), all from the colonies made the 31st July. The colonies will be put at 37°C on sunday 3rd in order to have grown colonies on monday.
D - Lemon scent
Results of the Transformation of electrocompetent cells
by Arnaud & Terry
Transformation of electrocompetent cells made the July 29th. Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)
Results:
- Nothing has grown on the control dishes.
- Failure, nothing has grown on the dishes which contain DY330 with pJBE1
Members there:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Romain, Sean and Terry.