Team:ULB-Brussels/Project/Methods

From 2014.igem.org

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Revision as of 16:54, 3 August 2014

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- Université Libre de Bruxelles -



Material and Methods


Some technical material used during our genetical experiments will be emphasized hereabouts.

Different kinds of experimental methods have been applied in our Lab too.

A selection of results obtained by our methods will be shown in the next section.

*

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Material:
A list of the Material is not yet accessible, but we hope this will be soon written. The usual equipment was: gloves, glasses and coat (especially because of UV emission & Ethidium Bromide during electrophoresis). There's more information in the page related with Safety.

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Methods:

First, birth and growing of bacteria populations (Centrifugation, Dilution).

Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis). Just after this step, bacteria selection (in function of the quantities od oligopeptids or phoA+prolin) and inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli.

Then, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and the principal properties of our bacteria (including Emission Spectroscopy) and analize of their genetical sequences.

Finally, conservation of bacteria populations producting the desired molecules or proteins in good quantities, at cold temperature in petri boxes and containers.

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