Team:Cambridge-JIC/Protocol

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<h4>PCR Protocol</h4>
<h4>PCR Protocol</h4>
<ol>
<ol>
-
<li> Add primer and template DNA to PCR tubes (label them) </li>
+
<li> Add primer (2.5µl) and template DNA (1µl) to PCR tubes (label them) </li>
   
   
<li> Create the phusion mix in a 1.5 ml eppendorf (note this is slightly  
<li> Create the phusion mix in a 1.5 ml eppendorf (note this is slightly  

Revision as of 11:45, 31 July 2014