Team:Paris Saclay/Notebook/July/23

From 2014.igem.org

(Difference between revisions)
(Wednesday 23rd July)
(Wednesday 23rd July)
Line 12: Line 12:
* BBa_J61051 - ''Salicylate promoter + NahR''
* BBa_J61051 - ''Salicylate promoter + NahR''
* BBa_K228001 - ''RNA suppressor''
* BBa_K228001 - ''RNA suppressor''
-
 
-
==Reunion==
 
-
 
-
Handled topics:
 
-
* Collaborations with Colombia, Paris Bettencourt and Virginia
 
-
* Organisation of the Meeting with french teams
 
-
* Definitions of the sub projects
 
-
* Definitions of notebook's standards
 
-
* Organisation of the Wiki
 
-
* Great discussion about Human Practices
 
===D - Lemon scent===
===D - Lemon scent===
Line 39: Line 29:
# Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span>
# Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span>
# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
 +
 +
==Reunion==
 +
 +
Handled topics:
 +
* Collaborations with Colombia, Paris Bettencourt and Virginia
 +
* Organisation of the Meeting with french teams
 +
* Definitions of the sub projects
 +
* Definitions of notebook's standards
 +
* Organisation of the Wiki
 +
* Great discussion about Human Practices
'''Members there''':
'''Members there''':

Revision as of 08:39, 31 July 2014

Contents

Wednesday 23rd July

Lab work

C - The Suppressing Salicylate System

Rehydration of BioBricks

by Fabio

The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution.

  • BBa_J61051 - Salicylate promoter + NahR
  • BBa_K228001 - RNA suppressor

D - Lemon scent

1 - p cola part 2

by Sean

p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.

Protocol

  1. Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
  2. If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was 150μl. Hence 150 μl of ethanol 100% was added, as well as 30μl of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
  3. Centrifuge for 10 minutes at 4°C and at 11000g.
  4. Discard supernatant.
  5. Add 1ml of ethanol 70%.
  6. Repeat step 3.
  7. Discard supernatant. Make tube interior as dry as possible. Tip: use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. Note: we decided to leave the tube at room temperature and continue tomorrow instead.
  8. (tomorrow) Resuspend pellet in 30μl H2O milliQ.

Reunion

Handled topics:

  • Collaborations with Colombia, Paris Bettencourt and Virginia
  • Organisation of the Meeting with french teams
  • Definitions of the sub projects
  • Definitions of notebook's standards
  • Organisation of the Wiki
  • Great discussion about Human Practices

Members there:

  • Instructors and advisors: Solenne and Sylvie.
  • Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.

Back to the calendar