Team:Paris Saclay/Notebook/July/23

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(Difference between revisions)
(→Wednesday 23rd July)
(→Wednesday 23rd July)
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==Lab work==
==Lab work==
-
 
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====1 - p cola part 2====
 
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''by Sean''
 
-
 
-
p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.
 
-
 
-
Protocol
 
-
# Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
 
-
# If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was '''150μl'''. Hence 150 μl of ethanol 100% was added, as well as '''30μl''' of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
 
-
# Centrifuge for 10 minutes at 4°C and at 11000g.
 
-
# Discard supernatant.
 
-
# Add 1ml of ethanol 70%.
 
-
# Repeat step 3.
 
-
# Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span>
 
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# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
 
===C - The Suppressing Salicylate System===
===C - The Suppressing Salicylate System===
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* Organisation of the Wiki
* Organisation of the Wiki
* Great discussion about Human Practices
* Great discussion about Human Practices
 +
 +
===D - Lemon scent===
 +
 +
====1 - p cola part 2====
 +
''by Sean''
 +
 +
p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.
 +
 +
Protocol
 +
# Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
 +
# If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was '''150μl'''. Hence 150 μl of ethanol 100% was added, as well as '''30μl''' of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
 +
# Centrifuge for 10 minutes at 4°C and at 11000g.
 +
# Discard supernatant.
 +
# Add 1ml of ethanol 70%.
 +
# Repeat step 3.
 +
# Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span>
 +
# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
'''Members there''':
'''Members there''':

Revision as of 08:39, 31 July 2014

Contents

Wednesday 23rd July

Lab work

C - The Suppressing Salicylate System

Rehydration of BioBricks

by Fabio

The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution.

  • BBa_J61051 - Salicylate promoter + NahR
  • BBa_K228001 - RNA suppressor

Reunion

Handled topics:

  • Collaborations with Colombia, Paris Bettencourt and Virginia
  • Organisation of the Meeting with french teams
  • Definitions of the sub projects
  • Definitions of notebook's standards
  • Organisation of the Wiki
  • Great discussion about Human Practices

D - Lemon scent

1 - p cola part 2

by Sean

p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.

Protocol

  1. Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
  2. If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was 150μl. Hence 150 μl of ethanol 100% was added, as well as 30μl of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
  3. Centrifuge for 10 minutes at 4°C and at 11000g.
  4. Discard supernatant.
  5. Add 1ml of ethanol 70%.
  6. Repeat step 3.
  7. Discard supernatant. Make tube interior as dry as possible. Tip: use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. Note: we decided to leave the tube at room temperature and continue tomorrow instead.
  8. (tomorrow) Resuspend pellet in 30μl H2O milliQ.

Members there:

  • Instructors and advisors: Solenne and Sylvie.
  • Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.

Back to the calendar

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