"
Team:Paris Saclay/Notebook/July/29
From 2014.igem.org
(Difference between revisions)
(Created page with "=Tuesday 29th July= ==Lab Work== '''Members there''': * Instructors and advisors: Alice, Solenne and Sylvie. * Students: Arnaud, Fabio, Romain, Sean and Terry. [http://2014.ig...") |
(→Tuesday 29th July) |
||
| Line 2: | Line 2: | ||
==Lab Work== | ==Lab Work== | ||
| + | |||
| + | ===A - The frame=== | ||
| + | |||
| + | ====PCR==== | ||
| + | ''by Romain'' | ||
| + | |||
| + | StrainS used: E. coli MG1655Z1 and E. coli MG1655. | ||
| + | |||
| + | Protocol: | ||
| + | |||
| + | *218µl of H20 MilliQ | ||
| + | *80µl GoTaq buffer 5X | ||
| + | *16µl oligonucleotide FTR-Apra-F (10mM) | ||
| + | *16µl oligonucleotide FTR-Apra-R (10mM) | ||
| + | *12µl DMSO 10mM | ||
| + | *32µl MgCl2 (25mM) | ||
| + | *8µl dNTP (10mM) | ||
| + | *2µl of GoTaq enzyme | ||
| + | *2µl of different bacterial cultures in each tubes: | ||
| + | |||
| + | #Tube 1: MG1655Z1 10I | ||
| + | #Tube 2: MG1655 100II | ||
| + | #Tube 3: MG1655 50I | ||
| + | #Tube 4: MG1655 100I | ||
| + | #Tube 5: MG1655Z1 10II | ||
| + | #Tube 6: MG1655 50II | ||
| + | #Tube 7: MG1655Z1 10I positive control with plasmid | ||
'''Members there''': | '''Members there''': | ||
Revision as of 09:56, 29 July 2014
Contents |
Tuesday 29th July
Lab Work
A - The frame
PCR
by Romain
StrainS used: E. coli MG1655Z1 and E. coli MG1655.
Protocol:
- 218µl of H20 MilliQ
- 80µl GoTaq buffer 5X
- 16µl oligonucleotide FTR-Apra-F (10mM)
- 16µl oligonucleotide FTR-Apra-R (10mM)
- 12µl DMSO 10mM
- 32µl MgCl2 (25mM)
- 8µl dNTP (10mM)
- 2µl of GoTaq enzyme
- 2µl of different bacterial cultures in each tubes:
- Tube 1: MG1655Z1 10I
- Tube 2: MG1655 100II
- Tube 3: MG1655 50I
- Tube 4: MG1655 100I
- Tube 5: MG1655Z1 10II
- Tube 6: MG1655 50II
- Tube 7: MG1655Z1 10I positive control with plasmid
Members there:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Romain, Sean and Terry.
