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Team:UCL/Diary/W2
From 2014.igem.org
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<li>In lab with Bez, Edo, Sanjay, and Dave. Prepared yet another fun restriction double digest for the 5 Lisbon plasmids, and 8 distribution BioBricks. Also digested the LacI //link// and RBS //link// in preparation for assembling/ligating our first BioBrick Device! (See Lab Notebook: 28/07/14. //link//)</li> | <li>In lab with Bez, Edo, Sanjay, and Dave. Prepared yet another fun restriction double digest for the 5 Lisbon plasmids, and 8 distribution BioBricks. Also digested the LacI //link// and RBS //link// in preparation for assembling/ligating our first BioBrick Device! (See Lab Notebook: 28/07/14. //link//)</li> | ||
<li>Had lunch in the quad with Bez and Edo, discussing a super-secret awesome HP idea that will help us win iGEM!</li> | <li>Had lunch in the quad with Bez and Edo, discussing a super-secret awesome HP idea that will help us win iGEM!</li> | ||
| - | <li>Writing my iGEM diary whilst relaxing on the quad pillars/steps under the hot hot sun. Probably time to move on elsewhere and do something more productive!</li> | + | <li>Writing my iGEM diary whilst relaxing on the quad pillars/steps under the hot hot sun. Probably time to move on elsewhere and do something more productive! Impromptu frisbee in the quad begins.</li> |
| - | <li></li> | + | <li><ul>Note to self: |
| + | <li>Email Brian our lab requirements.</li> | ||
| + | <li>Make RHnav scrolling.</li></ul></li> | ||
<li></li> | <li></li> | ||
</ol> | </ol> | ||
Revision as of 13:51, 28 July 2014
Contents |
Week 2
Monday
Lab meeting
- in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster!
- monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks//
- tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am).
- tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A
@YK need to email brian! what we need for lab space when we move
Edo
Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed.
Lewis M
Sanjay
Prepared restriction digests in the lab, pipetted so much I can barely type anymore. Watched Edo microwave his lunch. Our common room needs pillows so I can nap right now. Got some Bioprocess work done, and worked on the wiki. Good thing I can get drunk at the social tonight.
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
- Morning discussion about what we'll be doing this week at the lab meeting; primarily restriction digests to check what we have, so we're ready to ligate and assemble a BioBrick soon. With Lewis B, Soheila, Bez, Edo, Pamela, Dave, Sanjay, and Joy.
- In lab with Bez, Edo, Sanjay, and Dave. Prepared yet another fun restriction double digest for the 5 Lisbon plasmids, and 8 distribution BioBricks. Also digested the LacI //link// and RBS //link// in preparation for assembling/ligating our first BioBrick Device! (See Lab Notebook: 28/07/14. //link//)
- Had lunch in the quad with Bez and Edo, discussing a super-secret awesome HP idea that will help us win iGEM!
- Writing my iGEM diary whilst relaxing on the quad pillars/steps under the hot hot sun. Probably time to move on elsewhere and do something more productive! Impromptu frisbee in the quad begins.
- Note to self:
- Email Brian our lab requirements.
- Make RHnav scrolling.
Tanel
Adam
Kevin
Dave
Lewis B
Rob
Myriam
Alberto
Tuesday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Wednesday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Thursday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Friday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Weekend
